Coronavirus OC43 Lysate
Coronaviruses (CoV) are enveloped viruses with spiked projections, and contain a nucleocapsid with a positive-sense RNA. Coronavirus OC43, a Group 2 CoV, is propagated in the HCT-8 cell line. This virus is purified using sucrose density gradient ultracentrifugation, disrupted in the presence of 0.5% Triton X-100 non-ionic detergent/0.6 M KCl, and heat inactivated. Viral lysate…