Coronavirus NL63 Lysate
Coronaviruses (CoV) are enveloped viruses with spiked projections, and contain a nucleocapsid with positive-sense RNA. Coronavirus NL63, a Group 1 CoV, is propagated in the Vero cell line. This virus is purified using sucrose density gradient ultracentrifugation, disrupted in the presence of 0.5% Triton X-100 non-ionic detergent/0.6 M KCl, and heat inactivated. Viral lysate is…