This murine monoclonal antibody reacts with Human Immunodeficiency Virus Type 1 (HIV-1) p24 protein. The antibody was raised by immunizing BALB/c mice with purified HIV-1 (Strain IIIB) lysate. It is of the IgG1 subclass and has been purified from serum-free culture supernatant by protein G chromatography.

Anti-HIV-1 p24 (clone 39/5.1.23) is used to detect HIV-1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. This antibody exhibits reactivity with HIV-1 infected cultures using indirect immunofluorescence, exhibits reactivity with viral lysates examined using Western blotting. In addition, the antibody immunoprecipitates p24 protein from radiolabeled infected cell extracts. Antibody dilutions should be prepared using buffers containing suitable protein in order to stabilize antibody activity. Optimal dilution of antibody must be determined experimentally by the investigator.

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