Blastomyces Dermatitidis Recombinant W303-Bde, Genomic DNA
Each aliquot contains 1 ug of DNA extracted from a pure culture of a recombinant strain of Saccharomyces cerevisiae. A gene specific to Blastomyces dermatitidis was inserted into the S. cerevisiae genome using standard recombinant techniques. S. cerevisiae was confirmed by rDNA sequencing. The insert was detected with a specific in-house real-time PCR assay. The purity of the culture was monitored by additional culturing and Gram staining to detect any contaminating bacteria. The DNA was extracted from the cells following the bacterial protocol from the Qiagen Genomic DNA Handbook using Qiagen Genomic DNA Buffers with a 500/G or a 100/G genomic tip. This control is supplied in TE Buffer and should be frozen at -20°C or below. DNA concentration and 260/280 ratios are determined using a NanoDrop ND-1000.
Purified Genomic DNA is designed for use as an amplification and/or detection control for nucleic acid testing of B. dermatitidis. It can also be used to determine a limit of detection (LOD), in diagnostic assay development, cross-reactivity studies or genomic sequencing. Controls should be run using the same protocols as those used to amplify extracted clinical specimens. This control is intended to only be used with an assay from Luminex Molecular Diagnostics.