C. psittaci, an obligate intracellular bacteria, was propagated in a monolayer of L929 cells, harvested, titered, and inactivated using a combination of heat and chemical. The final titer reported was calculated based on a dilution of the pre-inactivation titer. The titer and infectivity of the harvested material were determined by coverslip culture of L929 cells in a 24-well plate followed by immunostaining with a genus-specific monoclonal antibody (Catalog No. 01-040000, Diagnostic Hybrids; USA).

Total Volume: 1.0 mL
Infectivity: No Growth
Final Titer (Calculated): 5.75 x 106 IFU/ml
Mycoplasma Contamination: Negative
Organism Verified by: PCR Specific Primers

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