Coxsackievirus Type B4 Lysate
Coxsackievirus is a non-enveloped enterovirus that contains a linear, positive-sense single-stranded RNA. Coxsackievirus Type B4 is propagated in the LLCmk2 cell line. This virus is purified using sucrose density gradient ultracentrifugation, disrupted in the presence of 0.5% Triton X-100 non-ionic detergent/0.6 M KCl, and heat inactivated. Viral lysate is usually sold in a vial containing 1.0 mg of protein, and is shipped on dry ice. Protein concentrations generally range from 0.5 to 3.0 mg/mL. Custom orders are available, including specific buffer formulations and package sizes.
This product is intended for research, product development, quality assurance testing, or further manufacturing use. Viral lysates can be utilized as an antigen, as a source for the purification of viral proteins, or for the detection of viral antibodies. Applications include: Immunodetection of antibodies to Coxsackievirus using solid-phase enzyme immunoassays (EIA); Western blot; Dot blot; Other protein-based assay.