Griffonia (Bandeiraea) simplicifolia Lectin (GSL II)
Griffonia simplicifolia lectin (GSL II) is isolated from a woody climbing shrub native to Africa and purified by affinity chromatography. The leaves and seeds from G. simplicifolia contain several different homologous lectins that vary in their carbohydrate specificities.GSL II is a tetramer that contains a single type of 30 kDa subunit and an isoelectric point between pH 5.0 and pH 6.0. It has insecticidal structure/function, the first GlcNAc binding legume lectin proven to have insecticidal activity.This lectin has a carbohydrate specificity for Gal and GalNAc, and elutes with galactose or N-acetylgalactosamine. Increasing the number of N-acetylglucosamine residues beyond two does not improve affinity. It has been reported to be unique in its ability to recognize exclusively – or -linked N-acetylglucosamine residues on the nonreducing terminal oligosaccharides. This lectin shows specificity for blood group A(-SA) greater than blood group B(-SA). See other GSL II conjugates.This product comes in a lyophilized form and is stored at -20° C. Calcium and manganese ions are required for binding.