Hippeastrum hybrid (Amaryllis) Lectin (HHL) – PureCheck our terms regarding shipping costs, taxes and duties
Hippeastrum hybrid lectin (HHL) isolated from amaryllis bulbs and purified by affinity chromatography. It consists of four subunits of identical size and has a molecular weight of 50, 000. HHL has an isoelectric point between pH 4.7 and pH 5.1. It only binds -mannose residues, (-1, 3) and (-1, 6) linked mannosyl units, as well as some yeast galactomannans, and elutes with the sugar MeMan. HHL has a blood group specificity for rabbit.Study shows when treatment of the linear -1, 3-mannan with periodate, resulting in oxidation of the terminal, nonreducing mannosyl group, reactivity with HHL was not reduced. Oligosaccharides containing either 1, 3- or 1, 6-linked mannosyl units are good inhibitors of HHL-mannan precipitation system. Observations suggests that HHL reacts not only with terminal but also with internal -D-mannosyl residues. Lectin appears to possess extended binding sites complementary to at least three 1, 6-linked -mannosyl units. Those containing -1, 3- or -1, 6-mannosyl residues were retarded on immobilized HHL agarose column. See other HHL conjugates.This product comes in a lyophilized form and is stored at -20° C. No metals ions are required for binding.