Hippeastrum hybrid (Amaryllis) Lectin (HHL) – Pure

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Hippeastrum hybrid lectin (HHL) isolated from amaryllis bulbs and purified by affinity chromatography. It consists of four subunits of identical size and has a molecular weight of 50, 000. HHL has an isoelectric point between pH 4.7 and pH 5.1. It only binds -mannose residues, (-1, 3) and (-1, 6) linked mannosyl units, as well as some yeast galactomannans, and elutes with the sugar MeMan. HHL has a blood group specificity for rabbit.Study shows when treatment of the linear -1, 3-mannan with periodate, resulting in oxidation of the terminal, nonreducing mannosyl group, reactivity with HHL was not reduced. Oligosaccharides containing either 1, 3- or 1, 6-linked mannosyl units are good inhibitors of HHL-mannan precipitation system. Observations suggests that HHL reacts not only with terminal but also with internal -D-mannosyl residues. Lectin appears to possess extended binding sites complementary to at least three 1, 6-linked -mannosyl units. Those containing -1, 3- or -1, 6-mannosyl residues were retarded on immobilized HHL agarose column. See other HHL conjugates.This product comes in a lyophilized form and is stored at -20° C. No metals ions are required for binding.

-20° C.

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