Human Immunodeficiency Virus Type 1 (HIV-1) Strain: IIIB Lysate
Human Immunodeficiency Virus Type 1 (HIV-1) is an enveloped retrovirus approximately 120 nm in diameter, which contains two copies of a singlestranded, positive-sense RNA. HIV-1 (Strain: IIIB) is propagated in the Hut78 T cell line. The virus is purified using sucrose density gradient ultracentrifugation, disrupted in the presence of 0.5% Triton X-100 non-ionic detergent/0.6 M KCl, and heat inactivated. HIV-1 lysate is sold in vials containing 1.0 mg of protein, and is shipped on dry ice. Protein concentrations generally range from 0.5 to 3.0 mg/ml. Custom orders are available, including specific buffer formulations and package sizes.
This product is intended for research, product development, quality assurance testing, or further manufacturing use.Viral lysates can be utilized as an antigen, as a source for the purification of viral proteins, or for the detection of viral antibodies. Applications include: Immunodetection of antibodies to HIV-1 using solid-phase enzyme immunoassays (EIA); Western blot; Dot blot; Other protein-based assays.
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