Lens culinaris lectin (LCA) is isolated from lentil seeds and purified by affinity chromatography. LCA is composed of four subunits that consists of an -chain (MW: 5.7 kDa) and -chain (MW: 17.5 kDa), with a total molecular weight of 46, 000. It has an isoelectric point between pH 7.6 and pH 8.4 and a carbohydrate specificity toward -D-mannose and -D-glucose, eluting with the sugar MeMan+MeGlc. This lectin is useful in affinity chromatography columns for the separation of glycoconjugates. The two isomers LCA-A and LCA-B agglutinates human red blood cells, although not blood group specific. Activity of LCA is determined by haemagglutination with human blood and agglutinates a 2% suspension of human erythrocytes at a lectin concentration of ?8g/ml. See other LCA conjugates.This product comes in a lyophilized form and is stable for more than three years when stored at -20?C. Calcium and manganese ions are required for binding.

-20° C.