Mouse Adiponectin glycosilated, HE (Recombinant)

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Recombinant Mouse Adiponectin HEK derived (Acrp30-HEK) Source: HEK293 (Human embryonic kidney cell line) Introduction: Adiponectin (also called ACRP30 and GBP28) is a protein exclusively secreted from adipose tissue. Specificity: The recombinant mouse adiponectin is 100% homologous with the Mouse serum adiponectin. Purity: Purity of recombinant mouse adiponectin is >98% (HPLC and SDS PAGE analyzed). Activity in vitro: Full-length adiponectin has been shown to activate AMP-activated protein kinase in hepatocyte (Yamauchi T. et al., 2002). It can also activate AMPK in HepG2 human hepatocytes at the concentration of as low as 1.0 g/ml. Activity in Vivo: Full-length adiponectin purified from mammalian cells can acutely decrease blood glucose levels (Berg A.H. et al., 2001). Single subcutaneous injection of adiponectin at the dose of 30 mg/g body weight significantly decreased the blood glucose levels in C57 mice. In vitro gluconeogenesis assay in primary rat hepatocytes was performed, showing the murine adiponectin derived from mammalian cells can inhibit glucose production. Effect of murine adiponectin on gluconeogenesis in primary rat hepatocytes. Primary rat hepatocytes were isolated and maintained as we described previously (Wang et al., 2002). The cells were then treated with 50 pM insulin plus different concentration of murine adiponectin purified from HEK293 cells for 48 hours. At the end of treatment, the cells were incubated with a glucose-free medium for 6 hours, and glucose production was measured as we described previously (n=4-6). Formulation: Sterile filtered and lyophilized from 0.5 mg/ml in TBS buffer. Reconstitution: Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely. Storage: Store lyophilized protein at -20 C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 C for a limited period of time; it does not show any change after two weeks at 4 C. Please avoid freeze-thaw cycles. Stability/Shelf Life: The lyophilized protein remains stable until the expiry date when stored at -20 C. APPLICATIONS: ELISA Western blotting cell culture and/or animal studies References: Xu A, Wang Y, Keshaw H, Xu LY, Lam KS, Cooper GJ. The fat-derived hormone adiponectin alleviates alcoholic and nonalcoholic fatty liver diseases in mice. J Clin Invest. 2003;112: 91-100. Wang Y, Xu A, Knight C, Xu LY, Cooper GJ. Hydroxylation and glycosylation of the four conserved lysine residues in the collagenous domain of adiponectin. Potential role in the modulation of its insulin-sensitizing activity. J Biol Chem. 2002;277: 19521-19529. Yamauchi T, Kamon J, Minokoshi Y, Ito Y, Waki H, Uchida S, Yamashita S, Noda M, Kita S, Ueki K, Eto K, Akanuma Y, Froguel P, Foufelle F, Ferre P, Carling D, Kimura S, Nagai R, Kahn BB, Kadowaki T. Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMPactivated protein kinase. Nat Med. 2002;8: 1288-1295. Berg AH, Combs TP, Du X, Brownlee M, Scherer PE. The adipocyte-secreted protein Acrp30 enhances hepatic insulin action. Nat Med. 2001;7: 947-953. Usage: products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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