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The IMMUNO-TEK Mouse IgG ELISA Kit is a rapid, easy to use enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse IgG in plasma, serum, hybridoma cell supernatants, ascites or other mouse biological fluids. The kit is especially useful in monitoring the production and purification of mouse monoclonal antibodies. The assay contains ready-to-use reagents and takes less than two hours to perform. The microplate and detector antibody in the kit have been specifically balanced to react uniformly with all subclasses of mouse IgG. The IMMUNO-TEK Mouse IgG ELISA Kit is for Research Purposes Only.

Microwells coated with polyclonal antibodies to mouse IgG form the capture phase of the assay. These antibodies bind uniformly to all subclasses of mouse IgG. Captured mouse IgG then reacts with detector antibody which is a polyclonal anti-mouse IgG conjugated with horseradish peroxidase. This reagent also reacts uniformly with all subclasses of mouse IgG. Enzyme activity in the wells are then quantified using tetramethyl benzidine as a substrate.

+2° to +8° C.
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