Myelin Oligodendrocyte Glycoprotein Human, Recombinant
Myelin Oligodendrocyte Glycoprotein, MOG, MOGIG-2, MGC26137.
Myelin Oligodendrocyte Glycoprotein is a membrane protein expressed on the oligodendrocyte cell surface and the outermost surface of myelin sheaths. Due to this localization, it is a prime target antigen that plays a role in immune-mediated demyelination. Myelin Oligodendrocyte Glycoprotein is involved in completion and maintenance of the myelin sheath and in cell-cell communication. MOG protein was found to differentially expressed in the dorsolateral prefrontal cortex and in the temporal lobe from patients with schizophrenia. MOG-specific antibody is crucial to the initiation of MOG-induced murine experimental autoimmune encephalomyelitis.
Myelin Oligodendrocyte Glycoprotein produced in E. coli is a single, non-glycosylated polypeptide chain containing a total of 132 amino acids (Met + 30-154 a.a. + 6x His tag at C-terminus) and having a total molecular mass of 15.2 kDa.
Sterile Filtered White lyophilized (freeze-dried) powder.
The Myelin Oligodendrocyte Glycoprotein 0.5mg/ml solution was lyophilized from 20mM sodium acetate buffer pH-4 and 0.3M sodium chloride.
It is recommended to reconstitute the lyophilized MOG in sterile 10mM Acetic acid not less than 100g/ml, which can then be further diluted to other aqueous solutions.
Lyophilized MOG although stable at room temperature for 3 weeks, should be stored desiccated below -18?C. Upon reconstitution MOG should be stored at 4?C between 2-7 days and for future use below-18?C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
5-20g per ml for In-Vitro Experiments and 50-100g per animal for In-Vivo study. The protein can be used for T-cell proliferation, cytokine induction, antigen presentation, western blotting, ELISA and EAE induction in mice.
Amino Acid Sequence
MGQFRVIGPRHPIRALVGDEVELPCRISPGKNATGMEVGWYRPPFSRVVHLYRN GKDQDGDQAPEY RGRTELLKDA IGEGKVTLRIRNV RFSDEGGFTCFFRDHSYQE EAAMELKVEDPFYWVSPGHHHHHH
Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
MOG, MOG Antibody