Phaseolus vulgaris (Kidney bean) Lectin (PHA-E+L) – Pure
Phaseolus vulgaris lectin (PHA E+L, also known as PHA P, P standing for phytohemagglutinin) is isolated from red kidney beans. It consists of two isolectins PHA-E and PHA-L, erythroagglutinin and leucoagglutinin, of which both possess strong mitogenic activity. PHA P has a molecular weight of 126, 000 and an isoelectric point between pH 5.2 and pH 6.2. It has a carbohydrate specificity for complex oligosaccharides and elutes with bovine thyroglobulin or acetic acid. This lectin agglutinates blood group A (-SA).PHA P can be used for mitogenic stimulation to human lymphocytes. It has the potential to induce closer contacts between adjacent cell membranes and has been successfully used for membrane-induced fusion in human oocytes, bovine oocyt, and caprine oocytes. PHA P is used for bacterial lectins in the initiation of infection, the basis for present attempts in academia and industry to apply carbohydrates for anti-adhesion therapy of such diseases. See other PHA E+L conjugates.This product comes in a lyophilized form and is stable for more than three years when stored below -20° C. Calcium and manganese ions are required for binding activity. This lectin is not soluble in distilled water and dilute buffers containing 10 , 15 mM NaCl solubilize it.