Styphnolobium japonicum (Japanese pagoda tree) Lectin (SJA) – Pure
Styphnolobium japonicum agglutinin (SJA) is isolated from Japanese pagoda tree seeds. It has a molecular weight of 133, 000 and consists of two subunits. The subunits can be separated into two subfractions, a D-galactose/N-acetyl-D-galactosamine specific lectin (B-SJA-I) and a D-mannose/D-glucose specific lectin (B-SJA-II). SJA has an isoelectric point between pH 4.9 and pH 5.6 and a carbohydrate specificity towards GalNAc. This lectin elutes with the sugar GalNAc.SJA agglutinates all blood group types but has greater affinity for A erythrocytes than of B types, than of O (-SA) types. Binding experiments of the lectin with frozen sections of human kidneys shows specific binding to the endothelia in specimens from blood groups B or AB, thus indicating a D-galactose/N-acetyl-D-galactosamine receptor specificity. SJA lacks mitogenic and immune-suppressive activity. See other SJA conjugates.This product comes in a lyophilized form and is stored at -20° C. Metal ions calcium and manganese are required for binding.