TBARS Assay kit
The sensitivity of measuring Thiobarbituric Acid Reactive Substances (TBARS) has made this assay the method of choice for screening and monitoring lipid peroxidation, a major indicator of oxidative stress(1-3). This rapid, easy-to-use procedure has been modified by researchers for use with many types of samples including drugs, food products and human and animal biological tissues(4-7). The assay has provided important information regarding free radical activity in disease states and has been used for measurement of anti-oxidant activity of several compounds(8-9). Although much controversy has appeared in the literature regarding the specificity of TBARS toward compounds other than MDA, it remains the most widely employed assay used to determine lipid peroxidation(10). If lipoprotein fractions are first acid precipitated from the sample, interfering soluble TBARS are minimized. Nonetheless, if TBARS are increased, it is recommended that a more specific assay such as HPLC be performed. The OXItek TBARS Kit is designed to provide a standardized, reproducible assay with consistent results. Each lot of reagents is quality controlled as a kit, which includes an MDA standard. It is recommended that additional in-house controls are included in each test run. The OXItek TBARS Assay Kit is for Research Purposes Only.
Store all kit reagents at 2-8Â°C. The components should be used before the expiration date indicated on the outside of the box.
+2° to +8° C.