TCGF (Natural T-Cell Growth Factor, IL-2)
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Each lot of IL-2/TCGF is analyzed for its ability to stimulate proliferation of seven to ten day old PHA-transformed human T- lymphocytes. When used at a final concentration of approximately 10% (v/v), IL-2/TCGF will induce a minimum fivefold increase in cell concentration of such cells when seeded at a density of 2.0 x 105/ ml. IL2/TCGF, a glycoprotein with a molecular weight of approximately 15,000 kDa, is produced from pooled human PHA-stimulated T-lymphocytes. IL-2/TCGF is purified by several chromatographic steps to remove PHA and interferon.
This preparation will support growth of most IL2/TCGF dependent lymphocyte cultures at a final concentration of 10% (v/v). The initial cell density should be approximately 2.0 x 105/ ml and the cell culture should be adjusted to this density every four to five days by additional feeding with 10% IL-2/TCGF. The initial cell population may need to be blast-transformed by either mitogenic or antigenic stimulation such as with PHA. Optimal concentration of IL-2/TCGF and/or mitogen must be determined experimentally by the investigator.