HIV-1 p24 Antigen ELISA Kit
The RETRO-TEK HIV-1 p24 Antigen ELISA is supplied for research purposes only. It is not intended for use in the diagnosis or prognosis of disease, or for screening and may not be used as a confirmatory test in diagnostic situations. The RETRO-TEK HIV-1 p24 Antigen ELISA is an enzyme linked immunoassay used to detect Human Immunodeficiency Virus Type 1 (HIV-1) p24 antigen in research specimens, including cell culture media, as well as human sera and plasma. It can also be used to monitor the purification and biochemical behavior of HIV-1. Furthermore, the assay may augment or supplant reverse transcriptase measurements traditionally employed to detect the presence of HIV-1. Such enzymatic measurements are not HIV-1 specific. The RETRO-TEK HIV-1 ICx/CRx Kit (ZMC Catalog n.: 0801096) for immune complex dissociation and confirmation of p24 reactivity enhances the detection of p24 antigen in serum or plasma. Many specimens contain HIV-1 viral loads greater than the range of the standard curve of this assay. The RETRO-TEK HIV-1 p24 Extended Range ELISA (ZMC catalog n. 0801137) will extend the standard curve of this assay to 4 ng/ml.
Microwells are coated with a monoclonal antibody specific for the p24 gag gene product of HIV-1. Viral antigen in the specimen is specifically captured onto the immobilized antibody during specimen incubation. The captured antigen is then reacted with a hightitered human anti-HIV-1 antibody conjugated with biotin. Following a subsequent incubation with Streptavidin-Peroxidase, color develops as the bound enzyme reacts with the substrate. Resultant optical density is proportional to the amount of HIV-1 p24 antigen present in the specimen.
+2° to +8° C.