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Chicken IgY ELISA

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Chicken IgY is the designation for the IgG-like molecule found in avian species. The IMMUNOtek Chicken IgY (IgG) ELISA is a rapid, easy to use enzyme linked immunosorbent assay (ELISA) designed for the measurement of chicken IgY in chicken serum, egg yolk or white, plasma, saliva, mucosa, cell culture fluid from chicken cells or other biological samples. Research Purposes Only. Not For in vitro Diagnostic Use.

Microplate wells coated with polyclonal antibodies reactive to Chicken IgY form the capture phase of the assay. Chicken IgY
specimen samples along with the supplied standard are diluted appropriately with Assay Diluent and are then incubated in the microplate wells. After a wash step, captured chicken IgY in the well is incubated with detector antibody, a polyclonal antichicken IgY conjugated to horseradish peroxidase (HRP). This antibody reacts only to the Fc portion of the immunoglobulin molecule allowing for the specific detection of IgY immunoglobulins. After another wash step, the chromogenic substrate tetramethyl benzidine (TMB) is added and a blue color develops in proportion to the amount of chicken IgY that has been bound to the antibody-coated plate. The enzyme reaction is stopped by the addition of Stop Solution which results in a color change to yellow that can be measured spectrophotometrically at 450 nm. The concentrations of chicken IgY are then calculated from a standard curve.

+2? to +8? C.
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