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Anti-CD36 Antibody [185-1 g2]

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Mouse monoclonal [185-1 g2] antibody to CD36.

Applications:Functional Studies, Flow Cytometry, IF, Host:Mouse, Clonality:Monoclonal, Clone ID:185-1 g2, Isotype:IgG2a, Conjugate:Unconjugated, Reactivity:Human, Immunogen:Stimulated human leukocytes, Specificity:This antibody recognizes a protein of 80kDa-90kDa, identified as CD36 (Workshop IV; Code P-26). Its epitope maps between aa155-183. It is expressed on platelets, monocytes and macrophages, microvascular endothelial cells, erythrocyte precursors, mammary epithelial cells, and some macrophage derived dendritic cells. CD36 acts as a receptor for thrombospondin (TSP), collagen types I, IV and V, P. falciparum malaria-infected erythrocytes, and sickle erythrocytes. It also functions as a scavenger receptor, mediating macrophage uptake of oxidized low-density lipoprotein (LDL) and recognition of apoptotic polymorphonuclear leukocytes (PMN). CD36 plays a role in platelet aggregation, macrophage foam cell development, inflammation, and the tissue ischemia observed in sickle cell disease and cerebral malaria. Note that 1-4% of Japanese and East Asia population lack CD36. This MAb blocks adhesion of P. falciparum parasitized red blood cells to CD36 and strongly inhibits collagen-induced platelet aggregation, Concentration:200 ?g/ml, Product form:Liquid, Molecular weight:80-90 kDa, Formulation:Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide, Light chain:kappa, Purification:Protein A/G chromatography, Storage:Shipped at 4?C. Upon delivery aliquot and store at -20?C. Avoid freeze / thaw cycles, Recommended dilutions:Flow Cytometry: 1-2 ?g/million cells, IF: 1-2 ?g/ml, Target:CD36

+25° C.


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