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Anti-CD8 alpha Antibody [KT15] (FITC)

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Rat monoclonal [KT15] antibody to CD8 alpha (FITC).

Applications:Flow Cytometry, Host:Rat, Clonality:Monoclonal, Clone ID:KT15, Isotype:IgG2a, Conjugate:FITC, Reactivity:Mouse, Immunogen:T cell clone, C6, Specificity:This antibody recognises the alpha chain of mouse CD8. CD8 is a heterodimeric protein composed of disulphide-linked CD8a and CD8? chains that is expressed primarily on cytotoxic T-cells. CD8 functions in the interaction with MHC Class I-bearing targets and plays a role in T-cell-mediated killing (Nakauchi , H. et al, 1985 & Nakauchi, H. et al, 1987).Clone KT15 is reported to block T-cell-mediated cytotoxicity in in vitro assays (Zeis, M. et al, 2002), Concentration:100 ?g/ml, Product form:Liquid, Formulation:Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide, Purification:Protein G affinity chromatography of tissue culture supernatant, Storage:Shipped at ambient temperature. Upon delivery aliquot and store at -20?C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4?C. Long term: store at -20?C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light, Recommended dilutions:Flow Cytometry: Neat for 0.1 mg, 1:50 for 0.5 mg – 1:10 for 0.1 mg, 1:100 for 0.5 mg, Use 10 ?l of the suggested working dilution to label 106 cells in 100 ?l.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B), Target:CD8 alpha

+25° C.


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