Cryptococcus Neoformans serotype D, DNA
Each aliquot contains 1 ug of DNA extracted from a pure culture of Cryptococcus neoformans. The identification of this organism was confirmed by rDNA sequencing. The purity of the culture was monitored by additional culturing and Gram staining to detect any contaminating bacteria. The DNA was extracted from the cells following a protocol based on the yeast protocol provided in the Qiagen Genomic DNA Handbook and using Qiagen Genomic DNA Buffers with a 500/G genomic tip. DNA concentration and A260/280 ratios are determined using a NanoDrop ND-1000. The extracted DNA also tested positive on an inhouse real time PCR assay.
Purified Genomic DNA is designed for use as an amplification and/or detection control for nucleic acid testing of Cryptococcus neoformans. It can also be used to determine a limit of detection (LOD), in diagnostic assay development, crossreactivity studies or genomic sequencing. When used as a control for nucleic acid tests, the same protocols as those used to amplify extracted clinical specimens should be employed.