Human IgG ELISA
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The IMMUNO-TEK Human IgG ELISA Kit is a rapid, easy to use enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human IgG in PREPARE REAGENT DILUTIONS serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. The kit is especially useful in monitoring the production and purification of human monoclonal antibodies. The assay contains ready-to-use reagents and takes less PIPETTE SPECIMENS AND STANDARDS than two hours to perform. The microplate and detector antibody in the kit have been specifically balanced to react uniformly with all subclasses of human IgG. The IMMUNO-TEK Human IgG ELISA Kit is for Research Purposes Only.
Microwells coated with polyclonal antibodies to human IgG form the capture phase of the assay. These antibodies bind uniformly to all subclasses of human IgG. Captured human IgG then reacts with detector antibody which is a polyclonal anti-human IgG PIPETTE DETECTOR ANTIBODY conjugated with horseradish peroxidase. This reagent also reacts uniformly with all subclasses of human IgG. Enzyme activity in the wells are then quantified using tetramethyl benzidine as a substrate
+2? to +8? C.