Unlock special prices! Log in or register to reveal exclusive offers.
01aaa_7839-20-1-jpg

Pneumocystis Jiroveci Recombinant W303-Pji, Genomic DNA

The prices will be displayed on the checkout.

Each aliquot contains 1 ug of DNA extracted from a pure culture of Saccharomyces cerevisiae. A gene specific to Pneumocystis jiroveci was inserted into the S. cerevisiae genome using standard recombinant techniques. S. cerevisiae was confirmed by rDNA sequencing. The insert was detected with a specific in-house real-time PCR assay. The purity of the culture was monitored by additional culturing and Gram staining to detect any contaminating bacteria. The DNA was extracted from the cells following a protocol based on the yeast protocol provided in the Qiagen Genomic DNA Handbook and using Qiagen Genomic DNA Buffers with a 500/G genomic tip. DNA concentration and A260/280 ratios are determined using a NanoDrop ND-1000. The extracted DNA also tested positive on an inhouse real time PCR assay.

Purified Genomic DNA is designed for use as an amplification and/or detection control for nucleic acid testing of P. jiroveci. It can also be used to determine a limit of detection (LOD), in diagnostic assay development, cross-reactivity studies or genomic sequencing. Controls should be run using the same protocols as those used to amplify extracted clinical specimens.

-20° C.
Product Insert.pdf

Material Safety Data Sheet.pdf

FacebookTwitterLinkedin

Additional Information

Product Code

Origin

USA

BTN - HC

Organism

Size

KGS

Shipping Conditions

0
    Your Cart
    Your cart is emptyReturn to Shop

    Unlock special prices!
    Log in or register to reveal exclusive offers.