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PON Standards contain human paraoxonase 1 (PON1) enzyme partially purified from human serum or plasma that has been phenotyped and genotyped for the Q192R polymorphism. PON Standards are suspended in buffer containing 25mM Tris-HCl, 0.17 mM NaCl, 20% glycerol, 0.1% Tergitol NP-10, 1.0 mM CaCl2, at pH 8.0. The PON1 enzyme activity is calcium-dependent and can be inhibited by chelating agents such as EDTA.
PON Standards are intended to be used as controls for arylesterase and paraoxonase applications, including kinetic activity assays and phenotype/genotype assays. For best results, dilute standards 1:3 for arylesterase assays and 1:20 for paraoxonase assays. Arylesterase activity reference ranges have been determined by the Zeptometrix Arylesterase / Paraoxonase Assay Kit (Catalog n. 0801199).