Rhizopus oryzae Z106, DNA
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Each aliquot contains 1 ug of DNA extracted from a pure culture of Rhizopus oryzae. The identification of this organism was confirmed by sequencing the ribosomal DNA locus. The purity of the culture was monitored by Lactophenol Cotton Blue staining, Gram staining and by additional culturing. The DNA was extracted from the cells following a custom protocol utilizing a FastPrep-24 and Qiagen QIAamp DNA Mini Kit protocols and reagents. This control is supplied in TE Buffer and should be frozen at -20°C or below. DNA concentration and 260/280 ratios are determined using a NanoDrop ND-1000. The extracted DNA also tested positive on an in-house real time PCR assay.
Purified Genomic DNA is designed for use as an amplification and/or detection control for nucleic acid testing of R. oryzae. It can also be used to determine a limit of detection (LOD), in diagnostic assay development, cross-reactivity studies or genomic sequencing. Controls should be run using the same protocols as those used to amplify extracted clinical specimens.