Preparation of microwell plates involve binding the antigen or antibody to the plate, blocking nonspecific binding sites, then coating the plate with a stabilizing reagent to allow for long term storage. ZeptoCoat is a polymeric immunoassay stabilization reagent designed to protect and extend the shelf life of immobilized antigen and antibody matrices applied to microwell plates. The reagent is supplied in a liquid, ready-to-use format.
ZeptoCoat is applied to stabilize adsorbed or immobilized proteins after the matrices have been bound to a microwell plate. ZeptoCoat may be used alone or in conjunction with a blocking solution. We recommend using a blocking agent such as ZeptoBlock (Catalog 0801185) to eliminate nonspecific binding of protein to the plate. Add 200 uL of ZeptoBlock or other blocking solution to each well and incubate at room temperature for 2 hours.
Aspirate the remaining solution and add 100 uL of ZeptoCoat to each well and incubate for 1 hour at room temperature. Aspirate the remaining solution and allow the plate to dry at room temperature for a minimum of 4 hours or overnight. Seal microwell plate in a bag with a desiccant and store at 2-8° C.
+2° to +8° C.
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