Western blot vs. Elisa: which of these two tests is the most accurate for HIV testing?

Western blot vs. Elisa: which of these two tests is the most accurate for HIV testing?

Post Views: 769 Today, advanced medical research and innovative molecular diagnostic technologies can efficiently control, treat and even cure many deadly diseases. Among them is the Human Immunodeficiency Virus, commonly known as HIV, which, as the name suggests, attacks our immune system. Often confused with AIDS (we debunk this myth in one of our previous…

Performance Assessment of Blood Screening Assays: Early Dynamics of Hepatitis B Virus (HBV)-DNA and Surface Antigen (HBsAg) in Ramp-Up Phase of Viremia

Performance Assessment of Blood Screening Assays: Early Dynamics of Hepatitis B Virus (HBV)-DNA and Surface Antigen (HBsAg) in Ramp-Up Phase of Viremia

Post Views: 914 In one of our previous articles, we talked about Hepatitis C, a serious liver disease caused by the hepatitis C virus (HCV) and exposed the severity of HCV and HIV co-infection. Today we discuss Hepatitis B, also a liver infection that is caused by the hepatitis B virus (HBV). Hepatitis B is…

ELISA vs western blot: a comparison of two common immunoassays

ELISA vs western blot: a comparison of two common immunoassays

Post Views: 1,652 In biomedical research, the word “immunoassay” is common terminology. That there are different types of immunoassays is common knowledge for someone working in a laboratory. What is an immunoassay?     Immunoassay is a technique that evaluates the presence and concentration of a macromolecule in a given solution with the help of…

Methods for purifying and analysing proteins via western blotting

Methods for purifying and analysing proteins via western blotting

Post Views: 2,865 For many years, the western blotting technique has been a primary method in molecular biology and techniques like proteomics (the large-scale study of proteomes: a set of proteins produced in an organism, system, or biological context). Given that western blot is a multistep process, often requires specialist interpretation and potential errors and…

Western Blot Procedures, Analysis and Purpose

Western Blot Procedures, Analysis and Purpose

Post Views: 16,155 Proteins play many vital roles in our bodies. Essentially proteins or polypeptides are complex compounds of amino acids that are needed by our bodies to regulate, function and construct organs and tissues in the body. Proteins are integral to organisms, helping almost every cell process and are also critical to metabolism. For…

An Overview of Simian immunodeficiency virus (SIV) and its impact

An Overview of Simian immunodeficiency virus (SIV) and its impact

Post Views: 4,084 What is the Simian immunodeficiency virus (SIV)? The Simian immunodeficiency virus is an HIV-like virus that can affect monkeys and apes and cause a disease similar to AIDS because HIV and SIV are closely related viruses. Researchers study SIV to explore HIV AIDS; however, SIV cannot infect humans and people with HIV…

Difference between line blots and Western blots

Difference between line blots and Western blots

Post Views: 8,151 What is western blot? Western blotting is also called Protein Immunoblotting because an antibody is mainly used to detect its antigen. It is a widely accepted analytical technique used to detect specific proteins in the given sample.  Western blot is generally used in research to separate and identify proteins. This technique separates…