Total Glutathione Peroxidase Assay Kit
Glutathione peroxidase (GPx, ECn. 188.8.131.52) is an enzyme found in cytoplasmic and mitochondrial fractions of cells. GPx acts on lipid hydroperoxide (LHP) substrates that are released from membrane phospholipids by phospholipase A2 . It can utilize cholesterol hydroperoxide (2) and hydrolyzes H2O2 at low concentrations(3). The antioxidant enzyme catalyzes the reduction of hydrogen peroxide and hydroperoxides formed from fatty acids, thereby effectively removing toxic peroxides from living cells. It plays the important role of protecting cells from potential damage by free radicals, formed by peroxide decomposition. (4, 5) The activity of GPx is coupled to glutathione reductase (GSSG-R), which maintains reduced glutathione (GSH) levels (6). Using glutathione (GSH) as a reducing reagent, the GPx enzymes catalyze the reduction of H2O2 and organic peroxides (R-O-O-H) to water and the corresponding stable alcohol thus inhibiting the formation of free radicals. Enzyme activity can be decreased by negative feedback from excess substrate or from damage by oxidative modification. (7) Oxidative stress has been implicated in aging and in the pathogenesis of a number of disorders. The extent of injury is generally related to an increase or decrease of one or more free radical scavenging enzymes of which GPx is one. (8) Various diseases show different levels of the universally present GPx in all tissues. A reduction in enzyme level is associated with Parkinsons disease (9) and in Chronic Glomerulonephritis patients. (10) In patients with end-stage renal disease (ESRD), GPx activity in adult patients was comparable to that in the control groups (children and adults); the GPx in children with ESRD was almost twice as high than in the other groups. (11) GPx activity decreased significantly in liver and increased in kidney in three-week-old diabetic rats that showed a reversal with a change in diet. (12) The OXItek Total Glutathione Peroxidase Assay Kit provides a method of quantifying the activity of total glutathione peroxidase. The Total Glutathione Peroxidase Assay Kit is for Research Purposes Only.
Store GPx-Reagent 1, GPx-Reagent 2 and GPx-Reagent 4 at -70Â°C. GPx-Reagent 3 and the GPx-Assay Buffer should be stored at 4Â°C.The components of the Kit are stable for 1 year when stored properly. The QC material when stored at -70Â°C is stable for 6 months unreconstituted.
+2Â° to +8Â° C.