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Anti-CD3 epsilon Antibody [CD3-12]

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Rat monoclonal [CD3-12] antibody to CD3 epsilon.

Applications:WB, IHC-P, IF, Flow Cytometry, IHC-Fr, Host:Rat, Clonality:Monoclonal, Clone ID:CD3-12, Isotype:IgG1, Conjugate:Unconjugated, Reactivity:Human, Bovine, Dog, Horse, Rhesus Monkey, Pig, Chicken, Mouse, Duck, Koala, Harbour Porpoise, Alpaca, Cynomolgus monkey, Spotted Hyena, Sea Lion, Cat, Amazon Parrot, Mammals, Birds, Raccoon, Great horned owl (Bubo virginianus), Bullfrog, Xenopus, Amphibia, Rabbit, African green monkey, Immunogen:Synthetic peptide sequence ERPPPVPNPDYEPC from cytoplasmic epitope of CD3, Specificity:This antibody recognises human CD3e. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (e, ?, d, ?) that assemble and function as three pairs of dimers (e?, ed, ??). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Malissen 2008; Guy and Vignali 2009; Smith-Garvin et al. 2009). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker.As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. (Jones et al. 1993; Kothlow et al. 2005), Concentration:1 mg/ml, Product form:Liquid, Formulation:Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide, Sequence:ERPPPVPNPDYEPC, Purification:Protein G affinity chromatography of tissue culture supernatant, Storage:Shipped at ambient temperature. Upon delivery aliquot and store at -20?C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4?C. Long term: store at -20?C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended, Recommended dilutions:FC 1: 1:50 – 1:100, IHC-Fr: 1:100, IHC-P 2: 1:100, Target:CD3 epsilon

+25° C.


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