Enterococcus Faecalis VRE, DNA
Each aliquot contains 10 ug of DNA extracted from a pure culture of Enterococcus faecalis. The identification of this organism was confirmed by 16S sequencing. The purity of the culture was monitored by Gram staining and by additional culturing. The DNA was extracted from the cells following a modified bacterial protocol from the Qiagen Genomic DNA Handbook using Qiagen Genomic DNA Buffers, Mini-Beadbeater-8 and 500/G genomic tips. This control is supplied in TE Buffer and should be frozen at -20°C or below. DNA concentration and 260/280 ratios are determined using a NanoDrop ND-1000. The extracted DNA also tested positive on an in-house real time PCR assay.
Purified Genomic DNA is designed for use as an amplification and/or detection control for nucleic acid testing of E. faecalis. It can also be used to determine a limit of detection (LOD), in diagnostic assay development, cross-reactivity studies or genomic sequencing. Controls should be run using the same protocols as those used to amplify extracted clinical specimens.