Rabbit IgG ELISA
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The IMMUNO-TEK Rabbit IgG ELISA Kit is a rapid, easy to use enzyme linked immunosorbent assay (ELISA) designed for the measurement of rabbit IgG in rabbit serum, plasma or other biological fluids. The assay contains ready-to-use reagents and takes less than two hours to perform. The microplate and detector antibody in the kit have been specifically balanced to react uniformly with all subclasses of rabbit IgG. The IMMUNO-TEK Rabbit IgG ELISA Kit is for Research Purposes Only.
Microplate wells coated with polyclonal antibodies to rabbit IgG form the capture phase of the assay. These antibodies bind uniformly to all subclasses of rabbit IgG. Captured rabbit IgG then reacts with detector antibody, which is a polyclonal anti-rabbit IgG, conjugated with horseradish peroxidase. This reagent also reacts uniformly with all subclasses of rabbit IgG. Enzyme activity in the wells is then quantified using tetramethyl benzidine as a substrate.
+2? to +8? C.