Phaseolus limensis (Lima bean) Lectin (LBA) – Alkaline phosphatase

Affinity-purified LBA is conjugated with alkaline phosphatase (AP). AP is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate molecule resulting in a colored or fluorescent product, or release of light as a chemiluminescent byproduct of the reaction. AP has optimal enzymatic activity at a basic pH between pH 8 and 10, and it’s reaction rate remains linear in which detection sensitivity can be improved by allowing the reaction to continue for a longer time.

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