Psophocarpus tetragonolobus (Winged bean) Lectin (PTL/PTA I- Galactose) – Pure
Psophocarpus tetragonolobus lectin (PTA/PTL I, psophocarpin B1, a storage protein with chymotrypsin inhibitory activity), is isolated from winged bean seeds and purified by affinity chromatography. The seed contains two lectins in which PTL I is the basic of the two, with an isoelectric point greater than pH 9.5 and a molecular weight of 58, 000. PTL I is a dimeric glycoprotein with specificity to GalNAc, with eluting sugar GalNAc. This lectin agglutinates trypsinized rabbit and human erythrocytes (type A and B) but not trypsinized human type O erythrocytes. PTL I recognizes blood group A-substance derived oligosaccharides such as trisaccharide GalNAc1-3Gal1-4Glc. Reports on the thermodynamics and kinetics of the lectin suggest that it has an extended binding site, and its interactions with saccharides involve reorientation of water molecules. See other PTL I conjugates.This product comes in a lyophilized form and is stored at -20° C.