Tulip sp. Lectin (TL) – Alkaline phosphatase
Affinity-purified TL is conjugated with alkaline phosphatase (AP). AP is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate molecule resulting in a colored or fluorescent product, or release of light as a chemiluminescent byproduct of the reaction. AP has optimal enzymatic activity at a basic pH between pH 8 and 10, and it’s reaction rate remains linear in which detection sensitivity can be improved by allowing the reaction to continue for a longer time.