Coronavirus 229E Lysate
Coronaviruses (CoV) are enveloped viruses with spiked projections, and contain a nucleocapsid with positive-sense RNA. Coronavirus 229E, a Group 1 CoV, is propagated in the HeLa or MRC-5 cell line. This virus is purified using sucrose density gradient ultracentrifugation, disrupted in the presence of 0.5% Triton X-100 non-ionic detergent/0.6 M KCl, and heat inactivated. Viral…