Human IgM ELISA
The IMMUNO-TEK Human IgM ELISA Kit is a rapid, easy to use enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human IgM in PREPARE REAGENT DILUTIONS serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. The kit is especially useful in monitoring the production and purification of human monoclonal antibodies. The assay contains ready-to-use reagents and takes less PIPETTE SPECIMENS AND STANDARDS than two hours to perform. The IMMUNO-TEK Human IgM ELISA Kit is for Research Purposes Only.
Microwells coated with polyclonal antibodies to human IgM form the capture phase of the WASH PLATE assay. Captured human IgM then reacts with detector antibody which is a polyclonal anti-human IgM conjugated with horseradish peroxidase. Enzyme activity in the wells are then quantified using tetramethyl benzidine as a substrate.
+2° to +8° C.