The way to Antibody Validation standards and its Challenges

The way to Antibody Validation standards and its Challenges

Post Views: 1,685 An Antibody is one of the most universally used reagents in science research, clinical tests and diagnostics. Regardless of their well-known use and the considerable investment of time and money that their benefits represent, no standard guidelines define how antibodies should be validated before use. What is Antibody validation? Validation is an…

Enzyme-linked Immunosorbent Assay Principle and Elisa Test Procedures.

Enzyme-linked Immunosorbent Assay Principle and Elisa Test Procedures.

Post Views: 9,778 Enzyme-linked immunosorbent assay (ELISA) is also known as an enzyme immunoassay (EIA). ELISA is defined as a biochemical technique used in many applications, including microbiology, blood screening, veterinary, and immunology, to detect antigens and antibodies present in a sample. ELISA technology is considered an essential diagnostic tool in plant and medicine pathology.…

Molecular and Cellular biology Applications of Lectin Conjugates

Molecular and Cellular biology Applications of Lectin Conjugates

Post Views: 2,078 Lectins are carbohydrate-binding proteins present in nearly all plants and a few animals as well. The word Lectins originated from a Latin word called ‘Legree,’ which means to select or pick. Lectins do not create any antigenic stimulation inside the immune system, but they do have the necessary capacity to bind analogously…

The Comprehensive Range of Monoclonal and Polyclonal antibodies against Infectious Agents

The Comprehensive Range of Monoclonal and Polyclonal antibodies against Infectious Agents

Post Views: 1,909 An antibody is a significant component hosting immune responses to viral pathogens, or other foreign invaders, such as chemicals, toxins, and pollens referred to as antigens. Antibodies can change to be highly specific to viral antigen-binding because of their unique maturation process. When an unknown substance invades the body, the immune system…

Range of Cytokines and Chemokines and its Clinical Significance

Range of Cytokines and Chemokines and its Clinical Significance

Post Views: 3,085 Cytokines and chemokines are referred to as immune-modulating agents that intercede and amend the responses of the system. The term “cytokine” is generated by two different Greek words: “cyto” meaning cell and “kinos” meaning movement. On the other hand, “chemokines” is a family unit of small cytokines, secreted by cells. Cytokines play…

Evaluation of the BioFire FilmArray Panel in Clinical Diagnostics

Evaluation of the BioFire FilmArray Panel in Clinical Diagnostics

Post Views: 2,618  The Biofire Filmarray System is an FDA, TGA, and CE-IVD, certified multiplex PCR system. It assimilates samples for detection, amplification, preparation, and analysis. It is a simple system that only requires a hands-on time of two minutes and a run time of an hour to get the fastest results in assisting hospitalized…

Applications of Nucleic Acid Testing in Molecular Diagnostics

Applications of Nucleic Acid Testing in Molecular Diagnostics

Post Views: 4,261 What is Nucleic Acid Testing? Nucleic acid amplification or Nucleic acid testing diagnosis is frequently referred to as NAT or NAAT. It is a technique that involves magnification and recognition of genetic material, the nucleic acids, and DNA, or RNA for diagnosis. Although all living beings’ genetic material is composed of RNA…

Difference between line blots and Western blots

Difference between line blots and Western blots

Post Views: 8,267 What is western blot? Western blotting is also called Protein Immunoblotting because an antibody is mainly used to detect its antigen. It is a widely accepted analytical technique used to detect specific proteins in the given sample.  Western blot is generally used in research to separate and identify proteins. This technique separates…

All you need to know about Oxidative stress and Inflammation

All you need to know about Oxidative stress and Inflammation

Post Views: 16,146 Oxidative stress occurs due to an imbalance between reactive oxygen species (ROS) production and the protective system’s ability to detoxify these reactive products. It can cause damage to cells and tissues and can lead to chronic inflammation. Long-term oxidative stress can also cause conditions such as diabetes, cancer, and heart disease. What…